Recombinant Rat Midkine Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35273
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 120 amino acids corresponding to Midkine Source: E. coli
Uniprot ID: Q9R1S9
Amino Acid Sequence: VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Midkine as determined by LAL method.
Predicted Molecular Mass
13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rat Midkine Protein
![SDS-PAGE: Recombinant Rat Midkine Protein [NBP2-35273]](https://resources.bio-techne.com/images/products/Rat-Midkine-Protein-SDS-Page-NBP2-35273-img0001.jpg "SDS-PAGE: Recombinant Rat Midkine Protein [NBP2-35273]")
SDS-PAGE: Recombinant Rat Midkine Protein [NBP2-35273]
SDS-Page: Rat Midkine Protein [NBP2-35273]Formulation, Preparation and Storage
NBP2-35273
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Midkine
Alternate Names
ARAP, MDK, MEK, MK1, NEGF2
Gene Symbol
MDK
Additional Midkine Products
Product Documents for Recombinant Rat Midkine Protein
Product Specific Notices for Recombinant Rat Midkine Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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