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Recombinant Human Nanog His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72370

Novus Biologicals, part of Bio-Techne
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NBP1-72370-250ug
NBP1-72370-50ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-154 of Human Nanog

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Nanog His Protein

Recombinant Human Nanog His Protein SDS-Page: Recombinant Human Nanog Protein [NBP1-72370]

SDS-Page: Recombinant Human Nanog Protein [NBP1-72370]

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72370
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Gene Symbol

NANOG

Additional Nanog Products

Product Documents for Recombinant Human Nanog His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Nanog His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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