Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35097
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 65 amino acids corresponding to Neuregulin-1 beta 1/NRG1 beta 1 Source: E. coli
Uniprot ID: Q02297
Amino Acid Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Neuregulin-1 beta 1/NRG1 beta 1 as determined by LAL method.
Predicted Molecular Mass
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Neuregulin-1 beta 1/NRG1 beta 1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein
![SDS-PAGE: Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein [NBP2-35097]](https://resources.bio-techne.com/images/products/Human-Neuregulin-1-beta-1-NRG1-beta-1-Protein-SDS-Page-NBP2-35097-img0001.jpg "SDS-PAGE: Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein [NBP2-35097]")
SDS-PAGE: Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein [NBP2-35097]
SDS-Page: Human Neuregulin-1 beta 1/NRG1 beta 1 Protein [NBP2-35097]Formulation, Preparation and Storage
NBP2-35097
| Formulation | Lyophilized from a 0.2 um filtered solution in 1 x PBS, pH 7.4. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Neuregulin-1 beta 1/NRG1 beta 1
Additional Neuregulin-1 beta 1/NRG1 beta 1 Products
Product Documents for Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein
Product Specific Notices for Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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