Recombinant Human NT-4 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35106
Key Product Details
Product Specifications
Description
A noncovalently linked homodimer of two 140 kDa polypeptide monomers (262 total amino acid residues) corresponding to NT-4 Source: E. coli
Uniprot ID: P34130
Amino Acid Sequence: MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of NT-4 as determined by LAL method.
Predicted Molecular Mass
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
NT-4 protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 104 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human NT-4 Protein
![SDS-PAGE: Recombinant Human NT-4 Protein [NBP2-35106]](https://resources.bio-techne.com/images/products/Human-NT-4-Protein-SDS-Page-NBP2-35106-img0001.jpg "SDS-PAGE: Recombinant Human NT-4 Protein [NBP2-35106]")
SDS-PAGE: Recombinant Human NT-4 Protein [NBP2-35106]
SDS-Page: Human NT-4 Protein [NBP2-35106]Formulation, Preparation and Storage
NBP2-35106
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 5.5. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: NT-4
Additional NT-4 Products
Product Documents for Recombinant Human NT-4 Protein
Product Specific Notices for Recombinant Human NT-4 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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