Recombinant Human OGG1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45318

Novus Biologicals, part of Bio-Techne
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NBP1-45318-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all OGG1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-345 of Human OGG1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Glycerol inhibits binding affinity to Ni-NTA beads in immunoprecipitation.

Application Notes

Use in In vitro assay reported in scientific literature (PMID 24489103).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human OGG1 His Protein

SDS-PAGE: Recombinant Human OGG1 His Protein [NBP1-45318]

SDS-PAGE: Recombinant Human OGG1 His Protein [NBP1-45318]

SDS-Page: Recombinant Human OGG1 His Protein [NBP1-45318] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-45318
Formulation PBS (pH 7.4), 40% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OGG1

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

8-oxoGuanine DNA Glycosylase

Alternate Names

8-hydroxyguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, AP lyase, DNA-apurinic or apyrimidinic site lyase, HOGG1, MMH, MUTMOGH1HMMH, N-glycosylase/DNA lyase

Gene Symbol

OGG1

Additional OGG1 Products

Product Documents for Recombinant Human OGG1 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human OGG1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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