Recombinant Human Osteoactivin/GPNMB His Protein

Name: Recombinant Human Osteoactivin/GPNMB His Protein
Brand: Novus Biologicals
SKU: NBP2-23082

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23082

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Osteoactivin/GPNMB Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 22-474 of Human Osteoactivin/GPNMB

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

53.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Osteoactivin/GPNMB His Protein

SDS-PAGE: Recombinant Human Osteoactivin/GPNMB His Protein [NBP2-23082]

SDS-Page: Recombinant Human Osteoactivin/GPNMB Protein [NBP2-23082]

Formulation, Preparation and Storage

NBP2-23082

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteoactivin/GPNMB

GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. The protein shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GPNMB protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Alternate Names

DC-HIL, GPNMB, HGFIN

Gene Symbol

GPNMB

Additional Osteoactivin/GPNMB Products

Product Documents for Recombinant Human Osteoactivin/GPNMB His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Osteoactivin/GPNMB His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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