Recombinant Rat PDGF-AA Protein

Name: Recombinant Rat PDGF-AA Protein
Brand: Novus Biologicals
SKU: NBP2-35281

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35281

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all PDGF-AA Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 111 amino acid polypeptide (A chain) chains corresponding to PDGF-AA Source: E. coli

Uniprot ID: P28576

Amino Acid Sequence: MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of PDGF-AA as determined by LAL method.

Predicted Molecular Mass

25.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PDGF-AA Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat PDGF-AA Protein

SDS-PAGE: Recombinant Rat PDGF-AA Protein [NBP2-35281]

SDS-Page: Rat PDGF-AA Protein [NBP2-35281]

Formulation, Preparation and Storage

NBP2-35281

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PDGF-AA

PDGF-AA is expressed by monocytes, macrophages, fibroblasts, and vascular smooth muscle and endothelial cells. It forms disulfide-linked homodimers as well as heterodimers with PDGF-B. PDGF-AA acts as a chemoattractant for multiple cell types. It signals through PDGF R alpha which is also the functional receptor for PDGF-AB, -BB, and -CC. PDGF-AA activity is modulated by the association of FGF R1 or Neuropilin-1 with PDGF R alpha.

Long Name

Platelet-derived Growth Factor AA

Alternate Names

PDGFAA

Gene Symbol

PDGFA

Additional PDGF-AA Products

Product Documents for Recombinant Rat PDGF-AA Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rat PDGF-AA Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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