Recombinant Human PDGF-D/SCDGFB His Protein

Name: Recombinant Human PDGF-D/SCDGFB His Protein
Brand: Novus Biologicals
SKU: NBP2-23335

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23335

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PDGF-D Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 250-370 of Human PDGF-D/SCDGFB

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSYHDR KSKVDLDRLN DDAKRYSCTP RNYSVNIREE LKLANVVFFP RCLLVQRCGG NCGCGTVNWR SCTCNSGKTV KKYHEVLQFE PGHIKRRGRA KTMALVDIQL DHHERCDCIC SSRPPR

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PDGF-D/SCDGFB His Protein

SDS-PAGE: Recombinant Human PDGF-D/SCDGFB His Protein [NBP2-23335]

SDS-Page: SCDGFB Protein [NBP2-23335]

Formulation, Preparation and Storage

NBP2-23335

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF-D

Platelet-derived growth factor D, also known as PDGFD, plays an important role in wound healing. This protein induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. This protein can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Recombinant human PDGFD protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Platelet-derived Growth Factor D

Alternate Names

PDGFD

Gene Symbol