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Recombinant Human Peroxiredoxin 4 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41141

Novus Biologicals, part of Bio-Techne
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NBP1-41141-0.5mg
NBP1-41141

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 38-271 of Human Peroxiredoxin 4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Peroxiredoxin 4 His Protein

SDS-PAGE: Recombinant Human Peroxiredoxin 4 His Protein [NBP1-41141]

SDS-PAGE: Recombinant Human Peroxiredoxin 4 His Protein [NBP1-41141]

SDS-Page: Recombinant Human Peroxiredoxin 4 Protein [NBP1-41141] - PRDX 4, 28.8 kDa (255aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-41141
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 4

PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

AOE372, PRDX4, Prx4

Gene Symbol

PRDX4

Additional Peroxiredoxin 4 Products

Product Documents for Recombinant Human Peroxiredoxin 4 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Peroxiredoxin 4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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