Recombinant Mouse Persephin Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35118
Key Product Details
Product Specifications
Description
A disulfide-linked homodimer of two 96 amino acid polypeptide chains corresponding to Persephin Source: E. coli
Uniprot ID: O70300
Amino Acid Sequence: ALAGSCRLWS LTLPVAELGL GYASEEKVIF RYCAGSCPQE ARTQHSLVLA RLRGRGRAHG RPCCQPTSYA DVTFLDDQHH WQQLPQLSAA ACGCGG
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Persephin as determined by LAL method.
Predicted Molecular Mass
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Persephin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35118
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 30 % ACN, 0.1 % TFA, 150 mM NaCl. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Persephin
Additional Persephin Products
Product Documents for Recombinant Mouse Persephin Protein
Product Specific Notices for Recombinant Mouse Persephin Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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