Recombinant Human PP4/PPP4C His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23383

Novus Biologicals, part of Bio-Techne
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NBP2-23383
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PP4 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-307 of Human PPP4C

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PP4/PPP4C His Protein

SDS-PAGE: Recombinant Human PP4/PPP4C His Protein [NBP2-23383]

SDS-PAGE: Recombinant Human PP4/PPP4C His Protein [NBP2-23383]

SDS-Page: Recombinant Human PPP4C Protein [NBP2-23383]

Formulation, Preparation and Storage

NBP2-23383
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP4

Serine/threonine-protein phosphatase 4 catalytic subunit, also known as PPP4C, belongs to the Serine/threonine-protein phosphatase catalytic subunits. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPP4C is a putative member of a novel PP family. The PPP4C may play a role in dephosphorylation and regulation of HDAC3. Recombinant human PPP4C protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Protein Phosphatase 4

Alternate Names

PP4C, PPH3, PPP4C, PPX

Gene Symbol

PPP4C

Additional PP4 Products

Product Documents for Recombinant Human PP4/PPP4C His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PP4/PPP4C His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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