Recombinant Human PPCDC His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49436

Novus Biologicals, part of Bio-Techne
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NBP1-49436-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PPCDC Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-204 of Human PPCDC

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PPCDC His Protein

SDS-PAGE: Recombinant Human PPCDC His Protein [NBP1-49436]

SDS-PAGE: Recombinant Human PPCDC His Protein [NBP1-49436]

SDS-Page: Recombinant Human PPCDC Protein [NBP1-49436] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-49436
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPCDC

PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CoaC, EC 4.1.1.36, FLJ14585, MDS018, phosphopantothenoylcysteine decarboxylase, PPC-DC

Gene Symbol

PPCDC

Additional PPCDC Products

Product Documents for Recombinant Human PPCDC His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PPCDC His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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