ProDots Recombinant Human BMP-4 Protein
R&D Systems, part of Bio-Techne | Catalog # PRD314
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD314 has been discontinued.
View all BMP-4 products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293 - Arg408
Ser293 - Arg408
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser293
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
22-25 kDa, reducing conditions
37-41, non-reducing conditions
37-41, non-reducing conditions
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is 2.5-15.0 ng/mL.
The ED50 for this effect is 2.5-15.0 ng/mL.
Scientific Data Images for ProDots Recombinant Human BMP-4 Protein
ProDots Recombinant Human BMP-4 Protein Bioactivity
ProDots®Recombinant Human BMP-4 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15.0 ng/mL.
ProDots Recombinant Human BMP-4 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing multiple bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in the gel are due to variable glycosylation.Formulation, Preparation and Storage
PRD314
| Reconstitution | For a stock solution, reconstitute at 50-200 μg/mL in sterile 4 mM HCl, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8°C under sterile conditions after reconstitution. • 3 months at -20 to -80°C under sterile conditions after reconstitution. |
Background: BMP-4
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
UniProt
Additional BMP-4 Products
Product Documents for ProDots Recombinant Human BMP-4 Protein
Product Specific Notices for ProDots Recombinant Human BMP-4 Protein
726251.1
For research use only
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