Recombinant Human Proteasome 20S alpha 5 His Protein

Name: Recombinant Human Proteasome 20S alpha 5 His Protein
Brand: Novus Biologicals
SKU: NBP1-78835

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78835

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Proteasome 20S alpha 5 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-241 of Human Proteasome 20S alpha 5

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

30.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Proteasome 20S alpha 5 His Protein

SDS-PAGE: Recombinant Human Proteasome 20S alpha 5 His Protein [NBP1-78835]

SDS-Page: Recombinant Human Proteasome 20S alpha 5 Protein [NBP1-78835] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-78835

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 10% glycerol, 100 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 5

PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proteasome subunit alpha type-5

Alternate Names

PSMA5

Gene Symbol