Recombinant Protein A His (C-Term) Cys (C-Term) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35014
Key Product Details
Source
E. coli
Tag
His (C-Term), Cys (C-Term)
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 307 amino acids corresponding to Protein A with a 6x His-tag and Cys on the C-terminus Source: E. coli
Uniprot ID: P02976
Amino Acid Sequence: MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Protein A as determined by LAL method.
Predicted Molecular Mass
34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to
Specificity
Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Protein A His (C-Term) Cys (C-Term) Protein
![SDS-PAGE: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]](https://resources.bio-techne.com/images/products/Protein-A-Cys-C-Term-His-C-Term-Protein-SDS-Page-NBP2-35014-img0001.jpg "SDS-PAGE: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]")
SDS-PAGE: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]
SDS-Page: Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]
Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]
![HPLC: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]](https://resources.bio-techne.com/images/products/Recombinant-Protein-A--Protein-HPLC-NBP2-35014-img0004.jpg "HPLC: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]")
HPLC: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]
HPLC: Recombinant Protein A Protein [NBP2-35014] - Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]Formulation, Preparation and Storage
NBP2-35014
| Formulation | Lyophilized from additive free solution. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with distilled water or saline. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Protein A
Alternate Names
PROA, Protein-A
Gene Symbol
SAOUHSC_00069
Additional Protein A Products
Product Documents for Recombinant Protein A His (C-Term) Cys (C-Term) Protein
Product Specific Notices for Recombinant Protein A His (C-Term) Cys (C-Term) Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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