Recombinant Human Protein Disulfide Isomerase/P4HB Protein

Name: Recombinant Human Protein Disulfide Isomerase/P4HB Protein
Brand: Novus Biologicals
SKU: NBP2-35195

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35195

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Protein Disulfide Isomerase/P4HB Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 502 amino acids (MRGSGSHHHHHH-PDI) corresponding to Protein Disulfide Isomerase/P4HB Source: E. coli

Amino Acid Sequence: MRGSGSHHHH HHAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Protein Disulfide Isomerase/P4HB as determined by LAL method.

Predicted Molecular Mass

56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Thiol Protein Reductase Activity is 0.001 change in 650nm/ min-2, determined by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase Activity is 0.5 umol active RNase A min-1 umol PDIA2, according to the re-activation of reduced and denatured RNase A.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Protein Disulfide Isomerase/P4HB Protein

SDS-PAGE: Recombinant Human Protein Disulfide Isomerase/P4HB Protein [NBP2-35195]

SDS-Page: Recombinant Human Protein Disulfide Isomerase/P4HB Protein [NBP2-35195] - Recombinant Human Protein Disulfide Isomerase/P4HB protein

Formulation, Preparation and Storage

NBP2-35195

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Recombinant Human Protein Disulfide Isomerase/P4HB Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Protein Disulfide Isomerase/P4HB Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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