Recombinant Human PTH Protein

Name: Recombinant Human PTH Protein
Brand: Novus Biologicals
SKU: NBP2-35212

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35212

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01270

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all PTH Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 34 amino acids corresponding to PTH Source: E. coli

Uniprot ID: P01270

Amino Acid Sequence: SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNF

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of PTH as determined by LAL method.

Predicted Molecular Mass

4.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Parathyroid Hormone Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PTH Protein

SDS-PAGE: Recombinant Human PTH Protein [NBP2-35212]

SDS-Page: Human PTH Protein [NBP2-35212]

Formulation, Preparation and Storage

NBP2-35212

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PTH

Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. It is a critical hormone in the regulation of Ca 2+ homeostasis, secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. The amino terminal (1-34) fragment of parathyroid hormone, called PTH (1-34) reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis.

Long Name

Parathyroid Hormone

Alternate Names

Parathormone, Parathyrin

Gene Symbol

PTH

UniProt