Recombinant Human PTH Protein

Name: Recombinant Human PTH Protein
Brand: Novus Biologicals
SKU: NBP2-35215

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35215

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01270

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all PTH Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 28 amino acids corresponding to PTH Source: E. coli

Uniprot ID: P01270

Amino Acid Sequence: LMHNLGKHLN SMERVEWLRK KLQDVHNF

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of PTH as determined by LAL method.

Predicted Molecular Mass

3.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PTH protein is fully biologically active when compared to standard. The specific activity is determined by UMR106 cell/cAMP method, corresponding to a specific activity of > 1.0 x 10^4 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PTH Protein

SDS-PAGE: Recombinant Human PTH Protein [NBP2-35215]

SDS-Page: Human PTH 7 - 34 a.a Protein [NBP2-35215]

Formulation, Preparation and Storage

NBP2-35215

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PTH

Parathyroid hormone is secreted by parathyroid cells. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). Most PTH is secreted in its intact, mature 9.5 kDa form, amino acids 1-84; however, it can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. No PTH fragment has been found to be the result of another gene or alternative splicing of the PTH gene.

Long Name

Parathyroid Hormone

Alternate Names

Parathormone, Parathyrin

Gene Symbol

PTH

UniProt