Native Rabbit Rabbit IgG, Fc

Source: Normal serum (Accession #:NP_000079.2)

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG F(c) fragment is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Multi-step

Application Notes

This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Protein / Peptide Type

Native Protein

Scientific Data Images for Rabbit IgG, Fc Native Protein

SDS-PAGE: Rabbit IgG, Fc Native Protein [NBP1-97061]

SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Formulation, Preparation and Storage

NBP1-97061

Preparation Method	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(c). No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	0.01% Sodium Azide
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rabbit IgG, Fc

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer possesses the epitope recognition site. Rabbit IgG F(c) fragment is ideal for investigators in Immunology, Cancer, and Microbiology research.

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Product Documents for Rabbit IgG, Fc Native Protein

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COA

SDS

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Product Specific Notices for Rabbit IgG, Fc Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Rabbit IgG, Fc Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97061

Key Product Details

Conjugate

Applications

View all Rabbit IgG, Fc Proteins and Enzymes »

Product Specifications

Description