Recombinant Human RAP1B His Protein

Name: Recombinant Human RAP1B His Protein
Brand: Novus Biologicals
SKU: NBP1-50971

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50971

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P61224

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rap1B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-181 of Human RAP1B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RAP1B His Protein

SDS-PAGE: Recombinant Human RAP1B His Protein [NBP1-50971]

SDS-Page: Recombinant Human RAP1B Protein [NBP1-50971] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-50971

Formulation	20 mM MES buffer (pH5.0), 0.2M NaCl, 5mM DTT, 20% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rap1B

RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Ras-related Protein 1B

Alternate Names

KREV, RAL1B

Gene Symbol

RAP1B

UniProt

P61224 (Human)

Additional Rap1B Products

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COA

Product Specific Notices for Recombinant Human RAP1B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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