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Recombinant Human RCAN3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23401

Novus Biologicals, part of Bio-Techne
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NBP2-23401

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 56-241 of Human RCAN3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RCAN3 His Protein

SDS-PAGE: Recombinant Human RCAN3 His Protein [NBP2-23401]

SDS-PAGE: Recombinant Human RCAN3 His Protein [NBP2-23401]

SDS-Page: Recombinant Human RCAN3 Protein [NBP2-23401]

Formulation, Preparation and Storage

NBP2-23401
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.2 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RCAN3

Calcipressin-3, also known as RCAN3, is a 241 amino acid protein that potentially is involved in central nervous system development. RCAN3 inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Overexpression of calcipressin-3 results in inhibition of calcineurin activity towards the nuclear factor of activated T-cells (NFAT) transcription factors and also downregulates NFAT-dependent cytokine gene expression in activated Jurkat T-cells. Highest expression occurs in heart, skeletal muscle kidney, liver and peripheral blood leukocytes. Recombinant human RCAN3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

Down syndrome candidate region 1-like 2, Down syndrome critical region gene 1-like 2, DSCR1L2regulator of calcineurin 3 isoform 13,45,MCIP3regulator of calcineurin 3 isoform 1a2,34,5, hRCN3, Myocyte-enriched calcineurin-interacting protein 3, RCAN family member 3, RCN3, Regulator of calcineurin 3, regulator of calcineurin 3 isoform 1a3,45,Down syndrome candidate region 1-like protein 2, regulator of calcineurin 3 isoform 1b2,34,5, regulator of calcineurin 3 isoform 1c2,34,5, regulator of calcineurin 3 isoform 1c3,45,calcipressin-3

Gene Symbol

RCAN3

Additional RCAN3 Products

Product Documents for Recombinant Human RCAN3 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human RCAN3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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