Recombinant C. perfringens a-N-acetylgalactosaminidase, CF

R&D Systems, part of Bio-Techne | Catalog # 5705-GH

R&D Systems, part of Bio-Techne
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5705-GH-100
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Key Product Details

Source

E. coli

Accession #

AAM55479

Conjugate

Unconjugated

Applications

Enzyme Activity

View all alpha-N-acetylgalactosaminidase/NAGA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived c. perfringens alpha-N-acetylgalactosaminidase/NAGA protein
Lys2-Lys619 with an N-terminal Met and 6-His tag
Accession # AAM55479

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

75 kDa

SDS-PAGE

65 kDa, reducing conditions

Activity

Measured by its ability to cleave alpha-N-acetylgalactosaminyl from 4-Nitrophenyl N-acetyl-alpha -D-galactosaminide.
The specific activity is >5,000 pmol/min/ug, as measured under the described conditions.

Formulation, Preparation and Storage

5705-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: alpha-N-acetylgalactosaminidase/NAGA

The ABO blood group system is the most important blood type system and alpha-N-acetylgalactoside is key to the ABO blood group antigen (1). alpha-N-acetylgalactosidase from Clostridium perfringens is a useful tool for removing alpha linked N-acetylgalactosamine from blood type A antigen to produce H antigen and blood type O (2, 3). Blood type O is universally compatible in the ABO system and is widely used in blood transfusion (2). The enzyme was highly selective for terminal N‑acetylgalactosamine residues (3). No other exoglycosidase activities, particularly neuraminidase, was detected. Recombinant alpha-N-acetylgalactosidase from Clostridium perfringens can potentially be used in enzymatic conversion of human blood type A red blood cells to universally transfusable type O red blood cells.

References

  1. Watkins, W.M. (1980) Adv. Hum. Genet. 10:1.
  2. Liu, Q.P. et al. (2007) Nature Biotechnol. 25:454.
  3. Calcutt, M. J. et al. (2002) FEMS Microbiol. Lett. 214:77.
  4. Hsieh, H.Y. et al. (2003) Protein Expr. Purif. 32:309.

Alternate Names

alphaNacetylgalactosaminidase, NAGA

Entrez Gene IDs

4668 (Human); 17939 (Mouse); 315165 (Rat)

Gene Symbol

NAGA

UniProt

AAM55479

Additional alpha-N-acetylgalactosaminidase/NAGA Products

Product Documents for Recombinant C. perfringens a-N-acetylgalactosaminidase, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant C. perfringens a-N-acetylgalactosaminidase, CF

For research use only

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