Recombinant Canine LIF Protein

R&D Systems, part of Bio-Techne | Catalog # 8600-LF

R&D Systems, part of Bio-Techne
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Carrier Free
8600-LF-025/CF
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With Carrier
8600-LF-025
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Key Product Details

Source

E. coli

Accession #

J9NRL6

Conjugate

Unconjugated

Applications

Bioactivity

View all LIF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived canine LIF protein
Pro24-Phe202

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro24

Predicted Molecular Mass

20 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.02-0.12 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8600-LF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8600-LF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

LIF (leukemia inhibitory factor) is a 32-62 kDa, variably glycosylated, monomeric member of the IL-6 family of helical cytokines (1-4). LIF is widely expressed and highly pleiotropic. It is important for many physiological processes including embryo implantation and development, hematopoiesis, bone metabolism, and inflammation (5). Canine LIF is synthesized as a 202 amino acid (aa) precursor protein with a 22 aa signal sequence that can be alternately spliced to produce a secreted (LIF-D), extracellular matrix-associated (LIF-M), or intracellular (LIF-T) isoform (6). LIF-D and LIF-M have identical 180 aa mature sequences (6). Mature canine LIF shares 91% sequence identity with human LIF, 80% sequence identity with mouse and rat LIF, and 91% identity with bovine and porcine LIF. LIF induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (LIF R) and a 130 kDa signal transducing subunit (gp130). The gp130 subunit does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. LIF has been shown to support embryo implantation and to promote immunosuppression during pregnancy (7, 8). During development, it regulates cell proliferation and differentiation. However, its ability to favor or oppose either of these events is cell and context dependent (4, 5). LIF is important in the proliferation and maintenance of canine embryonic stem cells and induced pluripotent stem cells (9-11). LIF is up-regulated in activated CD4+ T cells, promotes regulatory T cell differentiation, and inhibits Th17 cell function (2, 5, 12). In the brain, it is up-regulated by neuronal injury and promotes neuron survival and oligodendrocyte myelination (2, 4, 13). It is produced by the adrenal cortex and enhances adrenal production of cortisol and aldosterone (14). Tumor cell-derived LIF has been shown to promote the differentiation of monocytes into tumor-associated macrophages (15, 16). LIF also promotes endometrial remodeling and the differentiation of adipocytes and cardiac smooth muscle cells (2, 4, 5).

References

  1. Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. U S A 85:2623.
  2. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  3. Moreau, J.F. et al. (1988) Nature 336:690.
  4. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  5. Mathieu, M.E. et al. (2012) Stem Cell Rev. 8:1.
  6. Voyle, R.B. et al. (1999) Exp. Cell. Res. 249:199.
  7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  8. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. U S A 98:8680.
  9. Koh, S. et al. (2013) Stem Cells Dev. 22:951.
  10. Whitworth, D.J. et al. (2012) Stem Cells Dev. 21:2288.
  11. Luo, J. et al. (2011) Stem Cells Dev. 20:1669.
  12. Cao, W. et al. (2011) Immunity 35:273.
  13. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  14. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  15. Duluc, D. et al. (2007) Blood 110:4319.
  16. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

UniProt

J9NRL6

Additional LIF Products

Product Documents for Recombinant Canine LIF Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8600-LF (with carrier)
Download SDS 8600-LF/CF (carrier free)

Product Specific Notices for Recombinant Canine LIF Protein

For research use only

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