Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10071-AB

R&D Systems, part of Bio-Techne
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10071-AB-050
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Key Product Details

Source

CHO

Accession #

XP_005548871.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha/beta R2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived IFN-alpha/beta R2 protein
Cyno/Rhesus IFN-alpha R2
(Ile 27-Lys243)
Accession # XP_005548871.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile 27

Predicted Molecular Mass

51.4 kDa

SDS-PAGE

75 - 95 kDa, reducing conditions

Activity

Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity.
The ED50 for this effect is 0.500-3.50 μg/mL in the presence of 30 pg/mL of Recombinant Human IFN‑ beta (Catalog # 8499-IF).

Scientific Data Images for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Recombinant Cynomolgus IFN-alpha/beta R2 Fc Protein Inhibition Activity

Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein Inhibition Activity

Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Chimera (Catalog # 10071-AB) inhibits the anti-viral activity of (Catalog # 8499-IF) The ED50 for this effect is 0.500-3.50 µg/mL.
Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein SDS-PAGE

Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cyno/Rhesus IFN‑ alpha/ beta R2 Fc Chimera (Catalog # 10071-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Formulation, Preparation and Storage

10071-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha/beta R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL-10, and IL-28 families of cytokines (1, 2). Mature IFN-alpha / beta R2 consists of an extracellular domain (ECD), containing two fibronectin type III repeats, a transmembrane segment, and a cytoplasmic domain. Alternative splicing generates a secreted isoform that corresponds to the ECD as well as a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region (3, 4). The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit (5-7). The mature ECD of cynomolgus IFN-alpha/ beta R2 shares 93% and 100% amino acid (aa) sequence identity with that of human and rhesus, respectively. IFN-alpha/beta R2, in association with IFN-alpha/beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN-alpha and IFN-beta. IFN-alpha / beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (8, 9). IFN-alpha / beta R2 is also subject to presenilin-dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (10). High concentrations of soluble IFN-alpha / beta R2 bind and neutralize IFN-alpha and IFN-beta, while lower concentrations prolong the antiviral activity of circulating IFN-beta but not IFN-alpha (11). Human but not mouse IFN-alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (12).

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Lutfalla, G. et al. (1995) EMBO J. 14:5100.
  4. Novick, D. et al. (1995) J. Leukocyte Biol. 57:712.
  5. Pfeffer, L.M. et al. (1997) J. Biol. Chem. 272:11002.
  6. Gazziola, C. et al. (2005) Int. J. Oncol. 26:129.
  7. Kotenko, S.V. and S. Pestka (2000) Oncogene 19:2557.
  8. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  9. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  10. Saleh, A.Z.M. et al. (2004) Oncogene 23:7076.
  11. McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119.
  12. Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.

Long Name

Interferon alpha/beta Receptor 2

Alternate Names

IFN-aR2, IFN-bR2, IFNAR2, IFNbR2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Gene Symbol

IFNAR2

UniProt

XP_005548871.1

Additional IFN-alpha/beta R2 Products

Product Documents for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

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Product Specific Notices for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

For research use only

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