Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10058-CD

R&D Systems, part of Bio-Techne
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10058-CD-050
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Key Product Details

Source

HEK293

Accession #

Unique Sequence

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD155/PVR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD155/PVR protein
Cynomolgus Monkey CD155/PVR
(Asp28-Asn343)
Unique Sequence		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp28

Predicted Molecular Mass

85 kDa

SDS-PAGE

68-77 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human CD96v2 Fc Chimera  (Catalog # 9556-CD) that produces 50% optimal binding response is 0.25-1.25 μg/mL.   

Scientific Data Images for Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

Recombinant Cynomolgus CD155/PVR Fc Chimera Protein Binding Activity

Recombinant Cynomolgus CD155/PVR Fc Chimera Protein Binding Activity

When Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera (Catalog # 10058-CD) is coated at 5 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED50 of 0.25-1.25 µg/mL.
Recombinant Cynomolgus CD155/PVR Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus CD155/PVR Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-77 kDa and 140-150 kDa, respectively.

Formulation, Preparation and Storage

10058-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homophilicly (3, 4). The mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. The sequence of the cynomolgus CD155/PVR was isolated from a cynomolgus monkey cDNA library and aligns with the sequence for Macaca nemestrina (XP_011734201.1 aa24-339, T77M, V93T, G223S, R328G). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM‑1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM‑1 to endothelial cell CD155 promotes transendothelial migration (7).

References

  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4:73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  6. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Maier, M.K. et al. (2007) Eur. J. Immunol. 37:2214.
  9. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  10. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  11. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

UniProt

Unique Sequence

Additional CD155/PVR Products

Product Documents for Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

For research use only

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