Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11235-IR

R&D Systems, part of Bio-Techne
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11235-IR-100
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Key Product Details

Source

HEK293

Accession #

XP_005568119.1

Structure / Form

Disulfide-Linked Homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17RA/IL-17R Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-17RA/IL-17R protein
Cynomolgus Monkey IL-17RA
(Leu33-Trp320)
Accession # XP_005568119.1  		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu33

Predicted Molecular Mass

60 kDa

SDS-PAGE

93-103 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑0.500 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17 (Catalog # 7955-IL).

Scientific Data Images for Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF

Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein Bioactivity.

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑0.500 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17A Protein (7955-IL).

Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein (Catalog # 11235-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-103 kDa.

Formulation, Preparation and Storage

11235-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5).  Within the extracellular domain, cynomolgus monkey IL-17RA shares 95% sequence identity with human IL-17RA. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced up-regulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).

References

  1. Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
  2. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Yao, Z. et al. (1995) Immunity 3:811.
  5. Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
  6. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
  7. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  8. Toy, D. et al. (2006) J. Immunol. 177:36.
  9. McAllister, F. et al. (2005) J. Immunol. 175:404.
  10. Ye, P. et al. (2001) J. Exp. Med. 194:519.
  11. Schnyder, B. et al. (2005) Cytokine 31:191.
  12. Tan, W. et al. (2006) J. Immunol. 176:6186.
  13. Lubberts, E. et al. (2005) J. Immunol. 175:3360.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

XP_005568119.1

Additional IL-17RA/IL-17R Products

Product Documents for Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF

For research use only

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