Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 Fc, CF

R&D Systems, part of Bio-Techne | Catalog # 10759-LR

R&D Systems, part of Bio-Techne
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10759-LR-050
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Key Product Details

Source

CHO

Accession #

XP_005569951.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Lymphotoxin beta R/TNFRSF3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Lymphotoxin beta R/TNFRSF3 protein
Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3
(Ser28-Met227)
Accession # XP_005569951.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser28

Predicted Molecular Mass

48 kDa

SDS-PAGE

57-68 kDa, under reducing conditions

Activity

Measured by its ability to inhibit Lymphotoxin alpha1/ beta2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756.
The ED50 for this effect is 30.0-180 ng/mL in the presence of 100 ng/mL Recombinant Human Lymphotoxin  alpha1/ beta2 (Catalog # 8884-LY).

Scientific Data Images for Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 Fc, CF

Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 Fc Chimera Bioactivtiy.

Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 Fc Chimera (Catalog # 10759-LR) inhibits Lymphotoxin alpha1/beta2-induced IL-8 secretion in A375 human melanoma cells. The ED50 for this effect is 30.0-180 ng/mL in the presence of 100 ng/mL Recombinant Human Lymphotoxin alpha1/beta2 (8884-LY).

Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 Fc Chimera (Catalog # 10759-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-68 kDa and 114-136 kDa, respectively.

Formulation, Preparation and Storage

10759-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), previously called TNF RIII or TNF R‑related protein (TNF Rrp), is a type I transmembrane glycoprotein within the TNF receptor superfamily, designated TNFRSF3 (1‑3). Cynomolgus monkey and rhesus macaque LT beta R cDNA encodes 435 amino acids (aa) including a 30 aa signal peptide, a 197 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 187 aa cytoplasmic domain. The ECD contains four cysteine‑rich motifs characteristic of the TNF receptor superfamily (1, 2). Within the ECD cynomolgus monkey LTBR shares 65‑75% aa sequence identity with human, mouse, rat, canine, porcine, equine and bovine LT beta R. Soluble LT beta R can be formed by proteolytic cleavage of the ECD, and is an inhibitor of transmembrane LT beta R, as is recombinant LT beta R, which inhibits autoimmunity (3‑6). LT beta R is expressed by visceral, lymphoid, and other stroma, epithelia and myeloid cells, but not lymphocytes (2, 4). LT beta R ligands include homotrimers of LIGHT (TNFSF14; also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 (3, 4, 6). Depending on the cell type and expression of TRAF3, activation of LT beta R has been shown to induce canonical (IKK/RelA; pro‑inflammatory) or alternative (NIK/RelB; lymphoid organogenic) NF kappa B activation (6, 7). LT beta R is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures (3‑5, 8‑10). Secondary immune tissues are absent in LT beta R‑deficient mice (3-5). LT beta R engagement induces production of IL‑7, RANK, TRANCE/RANK L, VEGF‑C, adhesion molecules such as VCAM‑1, ICAM‑1 and MAdCAM, and chemokines such as CXCL13, CCL19 and CCL21 (3, 8 ‑ 10). LT beta R is expressed by hepatocytes, is up‑regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis (4, 6, 11). It regulates cell growth and can initiate inflammation‑related carcinogenesis (6, 11).

References

  1. Crowe, P.D. et al. (1994) Science 264:707.
  2. Force, W.R. et al. (1995) J. Immunol. 155:5280.
  3. McCarthy, D.D. (2006) Immunol. Res. 35:41.
  4. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
  5. Boehm, T. et al. (2003) J. Exp. Med. 198:757.
  6. Wolf, M.J. et al. (2010) Oncogene 29:5006.
  7. Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
  8. van de Pavert, S.A. et al. (2010) Nat. Rev. Immunol. 10:664.
  9. Mouri, Y. et al. (2011) J. Immunol. 186:5047.
  10. Vondenhoff, M.F. et al. (2009) J. Immunol. 182:5439.
  11. Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

XP_005569951.1

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 Fc, CF

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Product Specific Notices for Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 Fc, CF

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