Recombinant Cynomolgus Monkey Axl Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11144-AX

R&D Systems, part of Bio-Techne
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11144-AX-050
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Key Product Details

Source

CHO

Accession #

EHH59605.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Axl Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Axl protein
Cynomolgus Monkey Axl
(Glu33-Trp450)
Accession # EHH59605.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu33

Predicted Molecular Mass

72 kDa

SDS-PAGE

95-105 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Axl Fc Chimera (Catalog # 11144-AX) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human Gas6  (Catalog # 885-GSB) binds with an ED50 of 0.0700-0.700 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Axl Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey Axl Fc Chimera Protein Binding Activity.

When Recombinant Cynomolgus Monkey Axl Fc Chimera Protein (Catalog # 11144-AX) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human Gas6 (885-GSB) binds with an ED50 of 0.0700-0.700 ng/mL.

Recombinant Cynomolgus Monkey Axl Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey Axl Fc Chimera Protein (Catalog # 11144-AX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-105 kDa and 190-210 kDa, respectively.

Formulation, Preparation and Storage

11144-AX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature cynomolgus monkey Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, cynomolgus monkey Axl shares approximately 97% aa sequence identity with human Axl.  Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).

References

  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  4. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  5. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  6. Park, I.-K. et al. (2009) Blood 113:2470.
  7. Holland, S. et al. (2005) Cancer Res. 65:9294.
  8. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  9. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  10. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  11. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  12. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

EHH59605.1

Additional Axl Products

Product Documents for Recombinant Cynomolgus Monkey Axl Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey Axl Fc Chimera Protein, CF

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