Recombinant Cynomolgus Monkey B7-H2 His tagged Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9327-B7
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H2 protein
Asp19-Thr256, with a C-terminal 6-His tag
Asp19-Thr256, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp19
Predicted Molecular Mass
27 kDa
SDS-PAGE
54 - 70 kDa, reducing conditions
Activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3.
The ED50 for this effect is 0.15-0.9 μg/mL
The ED50 for this effect is 0.15-0.9 μg/mL
Scientific Data Images for Recombinant Cynomolgus Monkey B7-H2 His tagged Protein, CF
Recombinant Cynomolgus Monkey B7-H2 His tagged Protein Bioactivity
Recombinant Cynomolgus Monkey B7-H2 (Catalog # 9327-B7) co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL.Formulation, Preparation and Storage
9327-B7
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 200 μg/mL in PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: B7-H2
(2-6). B7-H2 binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14).In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15). B7-H2 and ICOS expressed on ILC2 cells. B7-H2/ICOS interaction promoted cytokine production and survival in ILC2 cells through STAT5, suggesting that B7-H2/ICOS signaling pathway is critically involved in ILC2 function and homeostasis (16).
References
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Wang, S. et al. (2000) Blood 96:2808.
- Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Faget, J. et al. (2012) Cancer Res. 72:6130.
- Yao, S. et al. (2011) Immunity 32:729.
- Watanabe, M. et al. (2008) J. Immunol. 180:5222.
- Wong, S.-C. et al. (2003) Blood 102:1381.
- Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
- Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
- Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
- Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
- Akbari, O. et al. (2002) Nat. Med. 8:1024.
- Her, M. et al. (2009) Lupus 18:501.
- Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
- Maazi H. et al. (2015) Immunity.42:538.
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Recombinant Cynomolgus Monkey B7-H2 His tagged Protein, CF
Product Specific Notices for Recombinant Cynomolgus Monkey B7-H2 His tagged Protein, CF
For research use only
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