Recombinant Cynomolgus Monkey CD4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10952-CD

R&D Systems, part of Bio-Techne
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10952-CD-100
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Key Product Details

Source

CHO

Accession #

P79185.1

Conjugate

Unconjugated

Applications

Bioactivity

View all CD4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD4 protein
Lys26-Pro396, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys26

Predicted Molecular Mass

42 kDa

SDS-PAGE

51-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When serially diluted Recombinant Cynomolgus Monkey CD4 His-tag is coated on the plate, it binds Human CD4 Biotinylated Antibody (Catalog # BAF379) with an ED50 of 25.0-250 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey CD4 His-tag Protein, CF

Recombinant Cynomolgus Monkey CD4 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey CD4 His-tag Protein (Catalog # 10952-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 51-60 kDa.

Formulation, Preparation and Storage

10952-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature cynomolgus monkey CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, cynomolgus monkey and human CD4 shares approximately 91% aa sequence identity. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells (3). CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is re-expressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

References

  1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
  2. Maddon, P.J. et al. (1985) Cell 42:93.
  3. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
  4. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
  5. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
  6. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
  7. Biswas, P. et al. (2003) Blood 101:4452.
  8. Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
  9. Funke, I. et al. (1987) J. Exp. Med. 165:1230.
  10. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
  11. Huppa, J.B. et al. (2010) Nature 463:963.
  12. Fragoso, R. et al. (2003) J. Immunol. 170:913.
  13. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
  14. Klatzmann, D. et al. (1984) Nature 312:767.
  15. Dagleish, A.G. et al. (1984) Nature 312:763.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

P79185.1

Additional CD4 Products

Product Documents for Recombinant Cynomolgus Monkey CD4 His-tag Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey CD4 His-tag Protein, CF

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