Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9246-CD

R&D Systems, part of Bio-Techne
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9246-CD-100
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Key Product Details

Source

HEK293

Accession #

NP_001271060

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIB/CD32b Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIIB/CD32b protein
Ala46-Pro217, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala46

Predicted Molecular Mass

20 kDa

SDS-PAGE

21-35 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Fc gamma RIIB/CD32b is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.3-1.5 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

Recombinant Cynomolgus Monkey Fc gamma RIIB Protein Bioactivity

Recombinant Cynomolgus Monkey Fc gamma RIIB Protein Bioactivity

When Recombinant Cynomolgus Monkey Fc gamma RIIB/CD32b (Catalog # 9246-CD) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50 of 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

9246-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The approximately 40 kDa mature cynomolgus Fc gamma RIIB consists of a 175 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 51 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIB shares 90%, 64%, and 61% aa sequence identity with human, mouse, and rat Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6).

References

  1. Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
  2. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  3. Takai, T. et al. (1996) Nature 379:346.
  4. Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
  5. Moll, T. et al. (2004) J. Immunol. 173:4724.
  6. Zhang, Y. et al. (2009) J. Immunol. 182:554.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Entrez Gene IDs

2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)

Gene Symbol

FCGR2B

UniProt

NP_001271060

Additional Fc gamma RIIB/CD32b Products

Product Documents for Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

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