Recombinant Cynomolgus Monkey Mer Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10576-MR

R&D Systems, part of Bio-Techne
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10576-MR-050
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Key Product Details

Source

CHO

Accession #

XP_005575320.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Mer Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Mer protein
Cynomolgus Monkey Mer
(Ala23-Ala501)
Accession # XP_005575320.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

79 kDa

SDS-PAGE

118-140 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Mer Fc Chimera (Catalog # 10576-MR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Protein S/PROS1  (Catalog # 9489-PS) binds with an ED50 of 2-10 μg/mL

Scientific Data Images for Recombinant Cynomolgus Monkey Mer Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey Mer Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey Mer Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Mer Fc Chimera Protein (Catalog # 10576-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 118-140 kDa and 236-280 kDa, respectively.

Formulation, Preparation and Storage

10576-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Tyrosine-protein Kinase Mer, also known as c-Mer and MerTK, is a member of the receptor tyrosine kinase subfamily TAM (Tyro3, Axl, and Mer). Mature cynomolgus Mer consists of 484 amino acid (aa) extracellular domain (ECD), a 20 aa transmembrane segment, and a 472 aa cytoplasmic domain. Within the ECD, cynomolgus Mer shares 95% and 99% sequence identity with human and rhesus monkey, respectively. Similar to Axl and Tyro3, the ECD of Mer contains two Ig-like motifs and two fibronectin type III motifs. Mer is not expressed in normal B- and T-cells but is expressed in neoplastic B- and T-cell lines (1-2).  It also shows higher expression in immunosuppressive M2‑like macrophages (3).  Mer is known to bind Gas6, Protein S, Tubby, Tubby-like protein 1 (Tulp1), and Galectin-3 (4-7). Binding of Gas6 lead to cell proliferation, migration or the prevention of apoptosis. Upon binding ligands via the Ig-like motif, Mer is dimerized to trans-autophosphorylate the kinase domain to induce downstream signaling. It has been shown that Mer signaling in macrophages induces M2 polarization, which promotes tumor growth, metastasis and evasion of anti-tumor immunity in tumor microenviroment (8). Inhibition of Mer, especially on leukocytes and macrophages, is an effective anti-cancer therapy (9).

References

  1. Graham, D.K. et al. (1994) Cell Growth Differ. 5:647.
  2. Graham, D.K. et al. (2006) Clin. Cancer Res. 12:2662.
  3. Shibata, T. et al. (2014) J. Immunol. 192:3569.
  4. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  5. Uehara, H. et al. (2008) J. Immunol. 180:2522.
  6. Caberoy, N.B. et al. (2010) EMBO J. 29:3898.
  7. Caberoy, N.B. et al. (2012) J. Cell Physiol. 227:401.
  8. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
  9. Cummings, C.T. et al. (2013) Clin. Cancer Res. 19:5275.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

XP_005575320.1

Additional Mer Products

Product Documents for Recombinant Cynomolgus Monkey Mer Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey Mer Fc Chimera Protein, CF

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