Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10718-PV

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10718-PV-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

HEK293

Accession #

XP_005549281.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PVRIG Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey PVRIG protein
Cynomolgus Monkey PVRIG
(Gly20, Ser21 or Glu23-Leu172)
Accession # XP_005549281.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20, Ser21, Glu23

Predicted Molecular Mass

45 kDa

SDS-PAGE

52-63 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey PVRIG Fc Chimera is immobilized at 1.00 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds with an ED50 of 0.150-0.750 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein Binding Activity.

When Recombinant Cynomolgus Monkey PVRIG Fc Chimera (Catalog # 10718-CV) is immobilized at 1.00 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 (2229-N2) binds with an ED50 of 0.150-0.750 μg/mL.

Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey PVRIG Fc Chimera (Catalog # 10718-PV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-63 kDa and 104-126 kda, respectively.

Formulation, Preparation and Storage

10718-PV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of cynomolgus monkey PVRIG have approximately 90% similarity with human PVRIG, respectively. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1-3). Blocking PVRIG with neutralizing antibody is a feasible approach for NK cell mediated immunotherapy of cancers (4, 5).

References

  1. Zhu, Y. et al. (2016) J. Exp. Med. 213:167.
  2. Whelan, S. et al. (2019) Cancer Immunol Res. 7:257.
  3. Murter, B. et al. (2019) Cancer Immunol Res. 7:244.
  4. Li, J. et al. (2020) Haematologica. DOI:10.3324/haematol.2020.258574. 
  5. Sanchez-Correa, B. et al. (2019) Cancers (Basel) 11:877.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Alternate Names

C7orf15, CD112R

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Gene Symbol

PVRIG

UniProt

XP_005549281.1

Additional PVRIG Products

Product Documents for Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.