Recombinant Cynomolgus Monkey uPAR His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10949-UK

R&D Systems, part of Bio-Techne
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10949-UK-100
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Key Product Details

Source

HEK293

Accession #

Q9GK78.1

Conjugate

Unconjugated

Applications

Bioactivity

View all uPAR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey uPAR protein
Leu23-Arg303, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

32 kDa

SDS-PAGE

45-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey uPAR His-tag protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human u‑Plasminogen Activator/Urokinase (Catalog # 1310-SE) binds with an ED50 of 20.0-120 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey uPAR His-tag Protein, CF

Recombinant Cynomolgus Monkey uPAR His-tag Protein Binding Activity.

When Recombinant Cynomolgus Monkey uPAR His-tag protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (1310-SE) binds with an ED50 of 12.0-120 ng/mL.

Recombinant Cynomolgus Monkey uPAR His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey uPAR His-tag Protein (Catalog # 10949-UK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-60 kDa.

Formulation, Preparation and Storage

10949-UK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a cysteine-rich cell surface glycoprotein belonging to the Ly6/uPAR (LU) superfamily and is involved in the activation of plasminogen to plasmin (1). Mature uPAR consists of an extracellular domain (ECD) with 3 LU domains (D1, D2, and D3) similar to Ly-6 antigens and snake venom alpha-neurotoxins and a C-terminal glycosyl-phosphatidylinositol (GPI) anchor linked to the last residue of the third LU domain (1-3). The ECD of mature cynomolgus uPAR shares 95% amino acid sequence identity with human uPAR. Posttranslational modification generates several soluble uPAR fragments including uPAR, uPAR DIIDIII, and uPAR DI in human (1-3). The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin. The binding of uPAR with uPA initiates a proteolytic cascade resulting in the degradation of extracellular matrix components and stimulates tissue remodeling (4). The uPAR/uPA interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis (4). uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site (5, 6). uPAR expression is limited in normal human tissue but is highly expressed by diverse cancer cells and by non-malignant cells that infiltrate cancers and uPAR expression is associated with poor prognosis and increased risk of metastasis (7). uPAR is considered as a biomarker in many inflammatory diseases including cancer, cardiovascular diseases, chronic kidney diseases and diabetes (6).

References

  1. Blasi, F. and Carmeliet, P. (2002) Nat Rev Mol. Cell Biol 3:932.
  2. Leth, J.M. and Ploug, M. (2021) Front Cell Dev. Biol. 9:732015.
  3. Behrendt, N. et al. (1996) J Biol. Chem. 271:22885.
  4. Mahmood, N. et al. (2018) Front. Oncol. 8:24.
  5. Smith, H. et al. (2010) Nat Rev Mol Cell Biol 11:23.
  6. Desmedt, S. et al. (2017) Crit. Rev. Clin. Lab. Sci. 54:117.
  7. Mazar, A.P. (2008) Clin. Cancer Res. 14:5649.

Long Name

Urokinase-type Plasminogen Activator Receptor

Alternate Names

PLAUR

Entrez Gene IDs

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Gene Symbol

PLAUR

UniProt

Q9GK78.1

Additional uPAR Products

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Product Specific Notices for Recombinant Cynomolgus Monkey uPAR His-tag Protein, CF

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