Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10807-VS

R&D Systems, part of Bio-Techne
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10807-VS-050
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Key Product Details

Source

HEK293

Accession #

XP_005593850.1

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey VSIG4 protein
Arg25-Pro288, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg25

Predicted Molecular Mass

30 kDa

SDS-PAGE

35-45 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.30-3.00 μg/mL

Scientific Data Images for Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF

Recombinant Cynomolgus Monkey VSIG4 His-tag Protein Bioactivity.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.30-3.00 μg/mL.

Recombinant Cynomolgus Monkey VSIG4 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey VSIG4 His-tag (Catalog # 10807-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa.

Formulation, Preparation and Storage

10807-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG4

VSIG4 (V-set and immunoglobulin domain containing 4), also known as CRIg and Z39IG, is a 45 kDa, type I transmembrane protein of the B7 family within the Ig superfamily that is expressed only in tissue-resident macrophages (1-4). The cynomolgus VSIG4 cDNA encodes 404 amino acids (aa) including a 24 aa signal sequence, a 264 aa extracellular domain (ECD) containing a V-type and a C2-type Ig domain, a 23 aa transmembrane domain and a 93 aa cytoplasmic domain (5). The cynomolgus VSIG4 ECD shares 94% aa identity with human VSIG4 ECD. VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-4, 6-9). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 6). It contributes significantly to innate immunity by binding and phagocytosis of complement-opsonized invading pathogens (4, 8, 10). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (10, 11). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappa B and thus be pro-inflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 7, 10, 11).

References

  1. He, J.Q. et al. (2008) Mol. Immunol. 4041.
  2. Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
  3. Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
  4. Helmy, K. et al. (2006) Cell 124:915.
  5. Entrez protein Accession # A0A2K5TW73, XP_005593850.
  6. Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
  7. Lee, M-Y. et al. (2006) J. Leukoc. Biol. 80:922.
  8. Gorgani, N.N. et al. (2008) J. Immunol. 181:7902.
  9. Walker, M.G. (2002) Biochim. Biophys. Acta 1574:387.
  10. Wiesmann, C. et al. (2006) Nature 444:217.
  11. Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.

Long Name

V-Set and Immunoglobulin Domain Containing 4

Alternate Names

Z39IG

Entrez Gene IDs

11326 (Human); 278180 (Mouse); 312102 (Rat); 102144784 (Cynomolgus Monkey)

Gene Symbol

VSIG4

UniProt

XP_005593850.1

Additional VSIG4 Products

Product Documents for Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF

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