Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10437-ZN

R&D Systems, part of Bio-Techne
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10437-ZN-020
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Key Product Details

Source

CHO

Accession #

XP_005579379.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PSMA/FOLH1/NAALADase I Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey PSMA/FOLH1/NAALADase I protein
Lys44-Ala750
with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

80 kDa

SDS-PAGE

94-105 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde.
The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein SDS-PAGE

Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey PSMA/FOLH1/NAALADase I His-tag (Catalog # 10437-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at ~100 kDa under reducing conditions.

Formulation, Preparation and Storage

10437-ZN
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PSMA/FOLH1/NAALADase I

Prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1,2). PMSA has a short cytosolic N-terminal domain, a single membrane-spanning segment, and an extracellular region that is composed of a protease domain, apical domain, and C-terminal domain (3). The extracellular domains all contribute to substrate recognition. The protein forms an active homodimer reliant on interactions between amino-acid side chains and glycosylation (3,4). PSMA is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, and N-acetylated-alpha-linked acidic dipeptidase-1 (NAALADase1). PSMA activity plays a role in tumor angiogenesis making it not only a tumor marker, but a therapeutic target in cancers including prostate cancer (5). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu (NAAG) to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity such as stroke, schizophrenia, Alzheimer's, and amyotrophic lateral sclerosis (6,7,8). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (8). Upregulation of PSMA is present in inflammatory bowel disease, Crohn's disease, and ulcerative colitis where pharmacological inhibition has shown amelioration of clinical symptoms pertaining to these diseases in mice (5).

References

  1. Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
  2. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
  3. Mesters, J.R. et al. (2006) EMBO J. 25:1375.
  4. Shulke, N. et al. (2003) Proc. Natl. Acad. Sci. USA. 100:12590.
  5. Vornov, J.J. et al. (2019) Neurochem. Res. [Epub ahead of print].
  6. Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
  7. Neale, J.J. and T. Yamamoto. (2020) Prog. Neurobiol. 184:101722.
  8. Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Gene Symbol

FOLH1

UniProt

XP_005579379.1

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

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Product Specific Notices for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

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