Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10225-IL

R&D Systems, part of Bio-Techne
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10225-IL-050
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Key Product Details

Source

CHO

Accession #

NP_001248216.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17F Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived IL-17F protein
Arg31-Gln163, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg31

Predicted Molecular Mass

16 kDa

SDS-PAGE

17-25 kDa, under reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 5-40 ng/mL

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF

Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein Bioactivity

Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein Bioactivity

Recombinant Cynomolgus Monkey/Rhesus Macaque IL-17F (Catalog # 10225-IL) induces IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-40 ng/mL.
Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus/Rhesus Macaque IL-17F His-tag (Catalog # 10225-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa and 30-50 kDa, respectively.

Formulation, Preparation and Storage

10225-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL-17) protein family, composed of six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL-17B which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Cynomolgus IL-17F cDNA encodes a 163 amino acid (aa) protein with a putative 30 aa signal peptide. Mature cynomolgus IL-17F shares 96% and 100% aa sequence identity with human and rhesus macaque IL-17F, respectively. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA-RE) have been identified. Although the ligands for IL-17 RD and RE are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL‑17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (3, 4). The biological activities mediated by IL-17F are similar to those of IL-17A. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (5). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (6).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  4. Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
  5. Hot, A. and Miossec, P. (2011) Ann. Rheum. Dis. 70:727.
  6. Starnes, T. et al. (2001) J. Immunol. 167:4137.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

NP_001248216.1

Additional IL-17F Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF

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