Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9996-N4

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R&D Systems, part of Bio-Techne
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9996-N4-050
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Key Product Details

Source

HEK293

Accession #

XP_005541277.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Nectin-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived Nectin-4 protein
Gly32-Ser349, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly32

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.1-1 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 9996-N4 in the following applications:

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF

Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein Bioactivity

Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein Bioactivity

Immobilized Recombinant Cynomolgus Monkey Nectin‑4 (Catalog # 9996-N4) supports the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-1 μg/mL.
Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Nectin‑4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-45 kDa.

Formulation, Preparation and Storage

9996-N4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4, also known as Poliovirus receptor-related protein 4 (Gene name: PVRL4), is a 66-kDa type I transmembrane glycoprotein belonging to the Nectin immunoglobulin superfamily (1). The Latin word necto means "to connect", indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1‑3). Human Nectin-4 is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain (1, 4, 5). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is up-regulated and a soluble 43-kDa form is found in the plasma (4-6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). Nectin extracellular domains (ECDs) contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (1, 3). Within the ECD, cynomolgus Nectin-4 shares 99%, and 91% amino acid sequence identity with human and mouse Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7). In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development (7). High Nectin-4/PVRL4 expression was associated with poor-prognosis in some invasive breast cancers and may be a new promising prognostic biomarker and specific therapeutic target (8).

References

  1. Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
  5. Takano, A. et al. (2009) Cancer Res. 69:6694.
  6. Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
  7. Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.
  8. M-Rabet, M. et al. (2017) Ann. Oncol. 28:769.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

XP_005541277.1

Additional Nectin-4 Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF

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