Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10960-NK

R&D Systems, part of Bio-Techne
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10960-NK-050
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Key Product Details

Source

CHO

Accession #

P61252.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all NKG2D/CD314 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived NKG2D/CD314 protein
MD
 Human IgG1
(Pro100-Lys330)

 IEGR Cynomologus Monkey NKG2D
(Trp74-Val216)
Accession # P61252.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met 

Predicted Molecular Mass

43 kDa

SDS-PAGE

45-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) binds with an ED50 of 25.0-200 ng/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein Binding Activity.

When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (AVI1300) binds with an ED50 of 25.0-200 ng/mL.

Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Formulation, Preparation and Storage

10960-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

Natural killer group 2, member D (NKG2D) is a type II lectin-like transmembrane glycoprotein is an activating receptor involved in both innate and adaptive immunities. Cynomologus NKG2D consists of an extracellular domain (ECD) with a C-type lectin domain, a transmembrane segment and a short cytoplasmic domain lacking signaling properties. The ECD lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands (1). The ECD of cynomologus NKG2D shares 98% amino acid sequence identity with the ECD of human NKG2D. NKG2D is expressed on CD8+ alpha beta T cells, gamma delta T cells, NK cells and NKT cells and additionally occurs on macrophages in mouse (1 - 3). The NKG2D receptor consists of a homodimer and has been shown to mediate immune responses and determine the activation status of NK cells (4). In human, ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3 (3, 5). Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection (6, 7). Alternative splicing of the NKG2D results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal (7). NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection (6, 8).

References

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Wang, J. et al. (2020) Biomol. 10:301.
  4. Garrity, D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:7641.
  5. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.

Long Name

Natural Killer G2D

Alternate Names

CD314, D12S2489E, KLRK1

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Gene Symbol

KLRK1

UniProt

P61252.1

Additional NKG2D/CD314 Products

Product Documents for Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

For research use only

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