Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11170-SL

Fc Chimera
R&D Systems, part of Bio-Techne
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11170-SL-050
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Key Product Details

Source

CHO

Accession #

XP_005541298.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all SLAM/CD150 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived SLAM/CD150 protein
Cynomolgus Monkey/Rhesus Macaque SLAM/CD150
(Tyr23-Lys236)
Accession # XP_005541298.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr23 & Thr25

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-80 kDa, under reducing conditions.

Activity

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.200-2.00 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 Fc Chimera Protein (Catalog # 11170-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 120-160 kDa, respectively.

Formulation, Preparation and Storage

11170-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM), also known as SLAMF1 and CD150, is the founding member of the SLAM subfamily of the CD2 protein family (1, 2). SLAM is a single-pass type I membrane glycoprotein that functions as an adhesion molecule and plays an active role in the regulation of innate and adaptive immunity (1, 2, 4). Mature SLAM consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a helical transmembrane domain, and a cytoplasmic tail containing 2 immunoreceptor tyrosine-based switch motifs (ITSM) (3, 4). The ECD of cynomolgus SLAM shares 97% amino acid identity with human SLAM. In human, several isoforms resulting from alternative splicing have been identified with functional diversity (4). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, and it is up-regulated on activated B cells and CD4+ and CD8+ T cells (4-6). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 phenotype in CD8+ T cells that is characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity (7, 8). SLAM also plays a role in activation of the PI3K-Akt signaling pathway through its association with the adapter molecule SAP (9). In humans, SLAM functions as a cellular entry receptor for measles virus (10, 11). SLAM deregulation is associated with genomic complexity and independently predicts a worse outcome in chronic lymphocytic leukemia (CLL) (12).

References

  1. Yurchenko, M. et al. (2018) J. Cell. Biol. 217:1411.
  2. Pellegrini, J. et al. (2021) Autophagy. 17:2629.
  3. Wang, N. et al. (2015) Front. Immunol. 6:158.
  4. Gordiienko, I. (2019) Clinical Immunol. 204:14.
  5. Calpe, S. et al. (2008) Adv Immunol. 97:177.
  6. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  7. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  8. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  9. Yurchenko, M.Y. et al. (2005) Exp Oncol. 27:24.
  10. Hsu, E.C. et al. (2001) Virology 279:9.
  11. Gonçalves-Carneiro, D. et al. (2017) J Virol. 91:e02255.
  12. Gian, M.R. et al. (2021) Br. J. Haematol. 192:1068.

Long Name

Signaling Lymphocytic Activation Molecule

Alternate Names

CD150, IPO-3, SLAMF1

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Gene Symbol

SLAMF1

UniProt

XP_005541298.1

Additional SLAM/CD150 Products

Product Documents for Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF

For research use only

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