Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6415-GH
Key Product Details
Product Specifications
Source
E. coli-derived e. coli alpha-Galactosidase/a-Gal protein
Val2-Val708, with an N-terminal Met and 6-His tag
Val2-Val708, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
82 kDa
SDS-PAGE
70-75 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6415-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: alpha-Galactosidase A/GLA
References
- Schmid, K. and Schmitt, R. (1976) Eur. J. Biochem. 67:95.
- Spangenberg, P. et al. (2000) Carbohydr. Res. 329:65.
Alternate Names
Agalsidase alpha, GALA, Melibiase
Entrez Gene IDs
2717 (Human)
Gene Symbol
GLA
UniProt
Additional alpha-Galactosidase A/GLA Products
Product Documents for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
Product Specific Notices for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
For research use only
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