Recombinant Equine FGF basic/FGF2/bFGF Protein

R&D Systems, part of Bio-Techne | Catalog # 8577-FB

R&D Systems, part of Bio-Techne
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Carrier Free
8577-FB-025/CF
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With Carrier
8577-FB-025
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Key Product Details

Source

E. coli

Accession #

E0AEV7

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF basic/FGF2/bFGF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived equine FGF basic/FGF2/bFGF protein
Pro10-Ser155

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro10 & Ala11

Predicted Molecular Mass

16 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 0.2-1 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8577-FB
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in water containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8577-FB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 or HBGF-2, is a widely expressed member of the fibroblast growth factor family (1-3). FGF basic functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain (2, 4-6). It is up-regulated in response to inflammatory stimuli such as TNF-alpha, IL-1 beta, IL-2, PDGF, and nitric oxide (2). The up-regulation of FGF basic in many human tumors is associated with tumor vascularity (2, 3). Among FGF family members, only FGF acidic and FGF basic lack signal peptides and are secreted by an alternative pathway. FGF basic is additionally localized in the nucleus where it regulates gene expression (2, 7). In humans, the use of alternative start sites can produce 21-23 kDa isoforms found only in the nucleus, and these isoforms target the expression of distinct genes (7). FGF family proteins share 35-60% amino acid (aa) identity. The 18 kDa equine FGF basic shares 97-99% amino acid  identity with bovine, canine, human, mouse, and rat FGF basic (8). FGF basic binds with picomolar affinity to FGF R1c and 2c (1, 2). Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alphav beta3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen,
alpha2-Macroglobulin, PDGF, and CXCL4/PF4 (2). These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones (2). In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors (1, 2).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  3. Grose, R. and C. Dickson (2005) Cytokine Growth Factor Rev. 16:179.
  4. Rosenblatt-Velin, N. et al. (2005) J. Clin. Invest. 115:1724.
  5. Reuss, B. et al. (2003) Cell Tissue Res. 313:139.
  6. Su, N. et al. (2008) Front. Biosci. 13:2842.
  7. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
  8. Abraham, J.A. et al. (1986) Science 233:545.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

UniProt

E0AEV7

Additional FGF basic/FGF2/bFGF Products

Product Documents for Recombinant Equine FGF basic/FGF2/bFGF Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8577-FB (with carrier)
Download SDS 8577-FB/CF (carrier free)

Product Specific Notices for Recombinant Equine FGF basic/FGF2/bFGF Protein

For research use only

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