Recombinant Feline Flt-3 Ligand/FLT3L Protein

R&D Systems, part of Bio-Techne | Catalog # 2467-FL

R&D Systems, part of Bio-Techne
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Carrier Free
2467-FL-025/CF
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With Carrier
2467-FL-025
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Key Product Details

Source

NS0

Accession #

NP_001009842

Conjugate

Unconjugated

Applications

Bioactivity

View all Flt-3 Ligand/FLT3L Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived feline Flt-3 Ligand/FLT3L protein
Ser27-Pro185, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

18.5 kDa

SDS-PAGE

25-33 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3.
The ED50 for this effect is 0.100-1.00 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2467-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2467-FL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature feline Flt-3 Ligand consists of a 156 amino acid (aa) extracellular region with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and an 88 aa cytoplasmic tail (4, 5). Within the ECD, feline Flt-3 Ligand shares 92%, 81%, 70%, and 63% aa sequence identity with canine, human, mouse, and rat Flt-3 Ligand, respectively. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 6). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates (6). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (6, 7). Multiple splice forms of canine, human, and mouse Flt-3 Ligand have been described and may also exist in feline (4, 6-10). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 5, 7, 10). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 11). Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (7, 8, 10). Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and -15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation (1, 12). Animal studies also show Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (13).

References

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Yang, S. and G.-K. Sim (2000) DNA Seq. 11:163.
  5. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  6. McClanahan, T. et al. (1996) Blood 88:3371.
  7. Hannum, C. et al. (1994) Nature 368:643.
  8. Lyman, S.D. et al. (1994) Blood 83:2795.
  9. Lyman, S.D. et al. (1995) Oncogene 10:149.
  10. Lyman, S.D. et al. (1993) Cell 75:1157.
  11. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  12. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  13. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

UniProt

NP_001009842

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Recombinant Feline Flt-3 Ligand/FLT3L Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 2467-FL (with carrier)
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Product Specific Notices for Recombinant Feline Flt-3 Ligand/FLT3L Protein

For research use only

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