Recombinant HIV-1 Gag p24 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11243-HV

E. coli-expressed
R&D Systems, part of Bio-Techne
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11243-HV-100
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Key Product Details

Source

E. coli

Accession #

NP_057850.1

Conjugate

Unconjugated

Applications

Bioactivity

View all HIV-1 Gag p24 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived viral HIV-1 Gag p24 protein
Pro149-Gly354, with a C-terminal 6-His tag and a N-terminal Met

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

24 kDa

SDS-PAGE

21-25 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant HIV-1 Gag p24 His-tag Protein, CF

Recombinant HIV-1 Gag p24 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HIV-1 Gag p24 His-tag Protein (Catalog # 11243-HV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-25 kDa.

Formulation, Preparation and Storage

11243-HV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, Urea with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load. This product is intended to be used to generate standard curves and/or positive controls for use in HIV-1 Gag p24 immunoassays.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

NP_057850.1

Additional HIV-1 Gag p24 Products

Product Documents for Recombinant HIV-1 Gag p24 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant HIV-1 Gag p24 His-tag Protein, CF

For research use only

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