Recombinant Human 5'-Nucleotidase/CD73 Fc Avi-tag, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11377

Fc Chimera
R&D Systems, part of Bio-Techne
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AVI11377-025
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Key Product Details

Source

HEK293

Accession #

AAH65937.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Enzyme Activity

View all 5'-Nucleotidase/CD73 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human 5'-Nucleotidase/CD73 protein
Human 5'-Nucleotidase/CD73
(Trp27-Lys547)
Accession # AAH65937.1		 GGIEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp27

Predicted Molecular Mass

86 kDa

SDS-PAGE

88-98 kDa, under reducing conditions

Activity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >5500 pmol/min/μg, as measured under the described conditions.

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human 5'-Nucleotidase/CD73 Fc Chimera Avi-tag (Catalog # AVI11377) binds Human/Equine 5'-Nucleotidase/CD73 Antibody (Catalog # MAB5795) with an ED50 of 3.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human 5'-Nucleotidase/CD73 Fc Avi-tag, CF

Biotinylated Recombinant Human 5'‑Nucleotidase/CD73 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human 5'-Nucleotidase/CD73 Fc Chimera Avi-tag Protein (Catalog # AVI11377) binds Human/Equine 5'-Nucleotidase/CD73 Antibody (MAB5795) with an ED50 of 3.00-30.0 ng/mL.

Formulation, Preparation and Storage

AVI11377
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: 5'-Nucleotidase/CD73

CD73, known as ecto-5'-Nucleotidase, converts extracellular nucleoside 5' monophosphates to nucleosides, with AMP as its preferred substrate (1). CD73 is a zinc-dependent, homodimeric enzyme bound to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor. It is composed of an N-terminal domain containing metal binding sites linked via small hinge region to a C-terminal domain containing the substrate binding site and dimerization interface (2). It is expressed by most cell types (3) and is widely expressed in tumor cell lines as well as upregulated in cancerous tissues (4, 5). CD73 is a key enzyme responsible for a rate-limiting step in the generation of extracellular adenosine. Adenosine is a molecule that signals through activation of purinergic receptors and results in an immunosuppressive role in the tumor microenvironment (4, 6). CD73 has been implicated in many pathological processes including immunomodulation and inflammation (7,8) tumor growth and metastasis (9-13) making CD73 a potential drug target in cancer. Targeting CD73 inhibition has resulted in numerous reports of favorable antitumor effects (4, 5, 12). Consequently, therapeutic approaches have been tested using knockdown, gene silencing and anti-CD73 therapies (11, 14) as well as small molecule inhibitors (14, 15).

References

  1. Zimmermann, H. et. al. (2012) Purinergic Signal. 8:437.
  2. Knapp, K. et. al. (2012) Structure. 20:2161.
  3. Resta, R. et. al. (1998) Immunol. Rev. 161:95. 
  4. Jin, D. et. al. (2010) Cancer Res. 70:2245.
  5. Zhang, B. (2010) Cancer Res. 70:6407.
  6. Picher, M. et. al. (2003) J. Biol. Chem. 278:13468. 
  7. Antonioli, L. et. al. (2012) Curr. Drug Targets 13:842.
  8. Eltzchig, H.K. et. al. (2012) N. Engl. J. Med. 367:2322.
  9. Bavaresco, L. et. al. (2008) Mol. Cell Biochem. 319:61.
  10. Yegutkin, G.G. et. al. (2011) Eur. J. Immunol. 41:1231.
  11. Stagg, J. et. al. (2012) Cancer Res. 72:2190.
  12. Ghalamfarsa, G. et. al. (2019) Expert Opin. Ther. Targets. 23:127.
  13. Gao, Z.W. et. al. (2014) Biomed. Res. Int. 2014:460654.
  14. Young, A. et. al. (2014) Cancer Discov. 4:879.
  15. McManus, J. et. al. (2018) SLAS Discov. 23:264.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Entrez Gene IDs

4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

Gene Symbol

NT5E

UniProt

AAH65937.1

Additional 5'-Nucleotidase/CD73 Products

Product Documents for Recombinant Human 5'-Nucleotidase/CD73 Fc Avi-tag, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human 5'-Nucleotidase/CD73 Fc Avi-tag, CF

For research use only

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