Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11415-EN

Fc Chimera
R&D Systems, part of Bio-Techne
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11415-EN-025
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Key Product Details

Source

HEK293

Accession #

AAH65937.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all 5'-Nucleotidase/CD73 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human 5'-Nucleotidase/CD73 protein
Human CD73
(Trp27-Ser549)
Accession # AAH65937.1		 GGIEGRMD Mouse IgG2a
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp27

Predicted Molecular Mass

85 kDa

SDS-PAGE

84-93 kDa under reducing conditions.

Activity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >12,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

11415-EN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: 5'-Nucleotidase/CD73

CD73, known as ecto-5'-Nucleotidase, converts extracellular nucleoside 5' monophosphates to nucleosides, with AMP as its preferred substrate (1). CD73 is a zinc-dependent, 70 kDa homodimeric enzyme bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. It is composed of an N-terminal domain containing metal binding sites linked via small hinge region to a C-terminal domain containing the substrate binding site and dimerization interface (2). It is expressed by most cell types (3) and is widely expressed in tumor cell lines as well as upregulated in cancerous tissues (4, 5). CD73 is a key enzyme responsible for a rate-limiting step in the generation of extracellular adenosine. Adenosine is a molecule that signals through activation of purinergic receptors and results in an immunosuppressive role in the tumor microenvironment (4, 6). CD73 has been implicated in many pathological processes including immunomodulation and inflammation (7, 8) tumor growth and metastasis (9-13) making CD73 a potential drug target in cancer. Targeting CD73 inhibition has resulted in numerous reports of favorable antitumor effects (4, 5, 12). Consequently, therapeutic approaches have been tested using knockdown, gene silencing and anti-CD73 therapies (11, 14) as well as small molecule inhibitors (14, 15).

References

  1. Zimmermann, H. et. al. (2012) Purinergic Signal. 8:437.
  2. Knapp, K. et. al. (2012) Structure. 20:2161.
  3. Resta, R. et. al. (1998) Immunol. Rev. 161:95. 
  4. Jin, D. et. al. (2010) Cancer Res. 70:2245.
  5. Zhang, B. (2010) Cancer Res. 70:6407.
  6. Picher, M. et. al. (2003) J. Biol. Chem. 278:13468. 
  7. Antonioli, L. et. al. (2012) Curr. Drug Targets 13:842.
  8. Eltzchig, H. K. et. al. (2012) N. Engl. J. Med. 367:2322.
  9. Bavaresco, L. et. al. (2008) Mol. Cell Biochem. 319:61.
  10. Yegutkin, G. G. et. al. (2011) Eur. J. Immunol. 41:1231.
  11. Stagg, J. et. al. (2012) Cancer Res. 72:2190.
  12. Ghalamfarsa, G. et. al. (2019) Expert Opin. Ther. Targets. 23:127.
  13. Gao, Z. W. et. al. (2014) Biomed. Res. Int. 2014:460654.
  14. Young, A. et. al. (2014) Cancer Discov. 4:879.
  15. McManus, J. et. al. (2018) SLAS Discov. 23:264.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Entrez Gene IDs

4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

Gene Symbol

NT5E

UniProt

AAH65937.1

Additional 5'-Nucleotidase/CD73 Products

Product Documents for Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF

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