Recombinant Human 5'-Nucleotidase/CD73 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5795-EN

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5795-EN-010
5795-EN-500
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

AAH65937

Structure / Form

Dimer

Conjugate

Unconjugated

Applications

Enzyme Activity

View all 5'-Nucleotidase/CD73 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human 5'-Nucleotidase/CD73 protein
Trp27-Lys547, with a C-terminal 6-His tag, Trp27-Lys547

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp27

Predicted Molecular Mass

59 kDa

SDS-PAGE

61-62 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 5795-EN in the following applications:

Formulation, Preparation and Storage

5795-EN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types (1). The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (2). CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. These functions include the adhesion of B-cells to follicular dendritic cells and T-cell signaling (3, 4). CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'-Nucleotidase activity (5). The native enzyme is a homodimer bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor.

References

    1. Resta, R. et al. (1998) Immunol. Rev. 161:95.
    2. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
    3. Airas, L. (1998) Leuk. Lymphoma 29:37.
    4. Resta, R. and Thompson. L.F. (1997) Cell Signal. 9:131.
    5. Jalkanen, S. and Salmi, M. (2008) Arterioscler. Thromb. Vasc. Biol. 28:18.

    Alternate Names

    5-NT, CD73, E5NT, eNT, NT5E, NTE

    Entrez Gene IDs

    4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

    Gene Symbol

    NT5E

    UniProt

    AAH65937

    Additional 5'-Nucleotidase/CD73 Products

    Product Documents for Recombinant Human 5'-Nucleotidase/CD73 Protein, CF

    Product Datasheets

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS

    Product Specific Notices for Recombinant Human 5'-Nucleotidase/CD73 Protein, CF

    For research use only

    Privacy and Cookie Policy
    Site Map
    © Copyright 2024 Bio-Techne. All Rights Reserved.