Recombinant Human 6-Phosphogluconate Dehydrogenase/PGD, CF
R&D Systems, part of Bio-Techne | Catalog # 8964-DH
Key Product Details
Product Specifications
Source
E. coli-derived human 6-Phosphogluconate Dehydrogenase/PGD protein
Ala2-Ala483, with a N-terminal Met and 6-His tag
Ala2-Ala483, with a N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
54 kDa
SDS-PAGE
48 kDa, reducing conditions
Activity
Measured by its ability to dehydrogenate 6-phosphogluconic acid.
The specific activity is >6000 pmol/min/μg, as measured under the described conditions.
The specific activity is >6000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
8964-DH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: 6-Phosphogluconate Dehydrogenase/PGD
6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH (2). Together with glucose-6-phosphate dehydrogenase (G6PD), these enzymes ensure the reducing environment of the cell cytoplasm (3). However, the activities of both enzymes are strongly inhibited by NADPH. It is not quite clear how the enzymes are regulated to maintain the level of reducing power inside cells (4). Deficiency of PGD is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of PGD and 6-phosphogluconolactonase suggest a synergism of the two enzymopathies (5).
References
- Barcia-Vieitez, R. and Ramos-Martinez, J.I. (2114) IUBMB Life 66:775.
- Wamelink, M.M. et al. (2008) J. Inherit. Metab. Dis. 31:703.
- Tsui, S.K.W. et al. (1996) Biochem. Genet. 34:367.
- Zimmer, H.G. (1996) Mol. Cell Biochem. 160-161:101.
- Caprari, P. et al. (2001) Ann. Hematol. 80:41.
Long Name
6-Phosphogluconate Dehydrogenase
Alternate Names
6PGD, PGDH
Gene Symbol
PGD
UniProt
Additional 6-Phosphogluconate Dehydrogenase/PGD Products
Product Documents for Recombinant Human 6-Phosphogluconate Dehydrogenase/PGD, CF
Product Specific Notices for Recombinant Human 6-Phosphogluconate Dehydrogenase/PGD, CF
For research use only
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