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Recombinant Human Active ASK1 (aa 649-946) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3575-KS

R&D Systems, part of Bio-Techne
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3575-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human ASK1 protein
aa 649-946

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

60 kDa

Activity

The specific activity of ASK1 is typically 82-112 nmol/min/mg using a myelin basic protein (MBP) substrate.

Scientific Data Images for Recombinant Human Active ASK1 (aa 649-946) Protein, CF

Recombinant Human Active ASK1 (aa 649-946) Protein SDS-PAGE

Recombinant Human Active ASK1 (aa 649-946) Protein SDS-PAGE.

The approximate molecular weight is 60 kDa and the purity is > 90%.

Formulation, Preparation and Storage

3575-KS
Formulation Supplied in 50 mM Tris HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: ASK1

ASK1, also known as MEKK5 or MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Over-expression of ASK1 induces apoptotic cell death and is activated in cells treated with tumor necrosis factor alpha (TNF ) (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References

  1. Ichijo, H. et al. (1997) Science 275:90.
  2. Nishitoh, H. et al. (1998) Mol. Cell 2:389.

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Entrez Gene IDs

4217 (Human); 26408 (Mouse)

Gene Symbol

MAP3K5

Additional ASK1 Products

Product Documents for Recombinant Human Active ASK1 (aa 649-946) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active ASK1 (aa 649-946) Protein, CF

For research use only

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