Recombinant Human Active Chk1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1630-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Chk1 protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
82 kDa
Activity
The specific activity of Chk1 was determined to be 228 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).
Scientific Data Images for Recombinant Human Active Chk1 Protein, CF
Recombinant Human Active Chk1 Protein SDS-PAGE.
The approximate molecular weight is 82 kDa and the purity is > 95%.Formulation, Preparation and Storage
1630-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Chk1
References
- Walworth, N. et al. (1993) Nature 363:368.
- Walworth, N.C. et al. (1996) Science 271:353.
Long Name
Checkpoint Kinase 1
Alternate Names
CHEK1
Gene Symbol
CHEK1
UniProt
Additional Chk1 Products
Product Documents for Recombinant Human Active Chk1 Protein, CF
Product Specific Notices for Recombinant Human Active Chk1 Protein, CF
For research use only
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